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用于测量血红蛋白的实验室方法是朗道试剂盒（血红蛋白氧化成高铁血红蛋白，然后用氰化钾，则总浓度与吸光度。反应）为血细胞比容是用实验室细胞比容离心机实验室方法。
贫血是在发展中国家非常普遍，在高精度的实验室方法往往无法使用的情况。检测贫血所以便携式方法已被开发出来。这些包括HEMOCUE（我们使用HEMOCUE Hb201），其测量血红蛋白和便携式血细胞比容（康盘100字段微型离心机），其测量含有红细胞的血液的比例。血细胞比容是也被称为收集细胞体积（PCV）和被测量为一百分比。然而，血红蛋白浓度的直接测量更加可靠，因为红细胞压积可能偶尔会脱水或利尿剂，它能够检测受损的血红蛋白浓度的能力混淆。
这个实验的目的是研究实验室方法和域之间的方法血红蛋白和红细胞压积的结果。二十血液样本（每个样品5毫升）从20名学生中收集了伦敦大学国王学院的营养学硕士2013-2014进行测试。There are laboratory and field methods to assess haemoglobin and haematocrit in blood. The laboratory method used to measure haemoglobin is Randox kit (haemoglobin is oxidized to methaemoglobin then reacts with potassium cyanide, then the total concentration is related to the absorbance.)Lab method for haematocrit is using laboratory haematocrit centrifuge.
Anaemia is very prevalent in developing countries, in situations where highly precise laboratory methods are often unavailable. So portable methods of detecting anaemia have been developed. These include the Hemocue (we use the Hemocue Hb201), which measures haemoglobin, and the portable haematocrit (Compur 100 field miicentrifuge), which measures the proportion of blood containing red cells. The haematocrit is also known as the Packed Cell Volume (PCV) and is measured as a percentage. However, direct measurement of haemoglobin concentration is more reliable since haematocritmay occasionally be confounded by dehydration or diuretics in its ability to detect an impaired haemoglobin concentration.
The aim of this experiment is to examine haemoglobin and haematocrit results between laboratory method and field method. Twenty blood samples(5ml per sample) were collected from 20 students in MSc Nutrition 2013-2014 of King’s College London for the test.
Fig. 1.Bland-Altman Plots of Haemoglobin Fig. 2.Bland-Altman Plots of Haematocrit
comparison between lab method and field mothodcomparisonbetween lab method and fieldmothod
Mean Bias - - - - - - -Limits of Agreement
Haemoglobin measured by field method is consistent with that measured by laboratory method which means the result of field method is reliable. haematocrit data from field method is significantly lower than that fromlaboratory method means field method for this is not that accurate.
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